rabbit anti gfap primary antibodies  (Proteintech)

Journal: Frontiers in Toxicology

Article Title: Promising protective potential of MiR-103a-3p against polystyrene microplastic neurotoxicity in rats

doi: 10.3389/ftox.2025.1560980

Figure Lengend Snippet: Histological analyses. (a) GFAP immunoreactivity in Hippocampus. (b) Nissl staining and statistical analysis of the number of Nissl-stained pyramidal neurons in the hippocampus. Data represent the mean ± SEM.

Article Snippet: Subsequently, the sections were subjected to an incubation period of 24 h with rabbit polyclonal anti-GFAP primary antibody (dilution 1/80) (SantaCruz Biotechnology, United States), under conditions of moisture and at an ambient temperature.

Techniques: Staining

Journal: Journal of Traditional and Complementary Medicine

Article Title: Elucidating the Mechanism of Traditional Chinese Medicine Formula (Yifei-sanjie Pill) in Alleviating the Chemobrain Based on Network Pharmacology and Experimental Verification

doi: 10.1016/j.jtcme.2024.11.008

Figure Lengend Snippet: Fig. 2. YFSJ reverses DDP-induced brain tissue damage. (A) Results of Nissl staining of the mouse hippocampus. The black arrow depicts the degenerative changes in the neurons in the DG sectors. Scale bar = 100 μm. (B) Effects of FJB staining on the hippocampus. Green fluorescence indicates degenerated neurons in the DG sectors. Scale bar = 100 μm. (C) Results of LFB staining of the hippocampus. The blank areas were stained to indicate myelin loss in neurons in the DG sectors. Scale bar = 200 μm. (D) GFAP and Iba1 immunostaining in the hippocampus. Merged overview of the relevant DG sectors with GFAP in red, Iba1 in green, and nuclear counterstaining with DAPI in blue. Scale bar = 50 μm.

Article Snippet: Primary antibodies against GFAP (Cat. #80788T), Iba1 (Cat. #17198T), Occludin (Cat. #91131T), Claudin-5 (Cat. #49564S), GPX4 (Cat. #59735S), and GAPDH (Cat. #5174T) and secondary antibodies anti-rabbit (Cat. #7074P2, 8889S and 4412S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Fluorescence, Immunostaining